human whole genome 4 × 44 k v2 microarray set  (Agilent technologies)

Journal: Translational Oncology

Article Title: Immune infiltration patterns and identification of new diagnostic biomarkers GDF10, NCKAP5, and RTKN2 in non-small cell lung cancer

doi: 10.1016/j.tranon.2023.101618

Figure Lengend Snippet: Basic information on the microarray datasets from the GEO database.

Article Snippet: The GPL10558 (Illumina HumanHT-12 v 4.0, expression beadchip), GPL13497 (Agilent-026,652 Whole Human Genome Microarray 4 × 44 K v2), GPL570 ([HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array), and GPL17077 (Agilent-039494 SurePrint G3 Human GE v2 8 × 60 K Microarray 039381) as detection platforms, and data from 113 NSCLC tissues and 69 adjacent normal tissues were collected from four mRNA microarray databases ( ).

Techniques: Microarray

Journal: Virology Journal

Article Title: Decoding pathogenesis factors involved in the progression of ATLL or HAM/TSP after infection by HTLV-1 through a systems virology study

doi: 10.1186/s12985-021-01643-8

Figure Lengend Snippet: Characteristics of datasets involved in the analysis and validation

Article Snippet: GSE55851 , Agilent-026652 Whole Human Genome Microarray 4 × 44 K v2 (GPL10332) , Normal: 3 ACs: 6 ATLL: 12.

Techniques: Expressing, Microarray

Journal: Scientific Reports

Article Title: Alterations of 63 hub genes during lingual carcinogenesis in C57BL/6J mice

doi: 10.1038/s41598-018-31103-3

Figure Lengend Snippet: Results of qRT-PCR and WES. (a) The expressions of mRNAs of Smad1 , Cebpa , Nfkb1 and Cyld are normalized to Gadph and calculated using the 2 −ΔΔCt method. The mRNA alterations of Smad1 , Cebpa , and Nfkb1 by qRT-PCR are identical to those determined by microarray. But the mRNA expression of Cyld by qRT-PCR presents upregulation and is opposite to those determined by microarray. ( b – d ) Chemiluminescent images of capillary with beta actin (b) and Cyld (c) had been assayed by Compass software individually and given as a lane vie (The images of C, M, and E lanes which were obtained in different experiments now are grouped and delineated with white space in b and c . The original images were supplied in supplementary information). The expression of Cyld was normalized by beta actin and was calculated at the value of Cyld divided by that of beta actin in each sample. The results of WES were evaluated using analysis of variance ( d , N = 5; error bars represent ± standard deviation). The result demonstrates that Cyld is statistically significantly down-regulated between C, M, and E (ANOVA, α = 0.05, F = 15.58, P < 0.01). C1–C5: samples from animals of 0th week. M1–M5: samples from animals of 12th week. E1–E5: samples from animals of 28th week.

Article Snippet: After amplified and labeled, Total RNA was hybridized onto a Whole Mouse Genome Oligo Microarray (4 × 44 K, v2, Agilent).

Techniques: Quantitative RT-PCR, Microarray, Expressing, Software, Standard Deviation